Discovery and biosynthesis of bacterial drimane-type sesquiterpenoids from Streptomyces clavuligerus

• Dongxu Zhang,
• Wenyu Du,
• Xingming Pan,
• Xiaoxu Lin,
• Fang-Ru Li,
• Qingling Wang,
• Qian Yang,
• Hui-Min Xu and
• Liao-Bin Dong

Beilstein J. Org. Chem. 2024, 20, 815–822, doi:10.3762/bjoc.20.73

• analogs. This discovery not only broadens the known chemical diversity of DMTs from bacteria, but also provides new insights into DMT biosynthesis in bacteria. Keywords: bacterial terpenoid; cytochrome P450s; drimane-type sesquiterpenoid; Streptomyces clavuligerus; terpenoid biosynthesis; Introduction

Bacterial terpene biosynthesis: challenges and opportunities for pathway engineering

• Eric J. N. Helfrich,
• Geng-Min Lin,
• Christopher A. Voigt and
• Jon Clardy

Beilstein J. Org. Chem. 2019, 15, 2889–2906, doi:10.3762/bjoc.15.283

• genome does not guarantee their partnership [87]. Studies of CYPs in bacterial terpenoid biosynthesis lags behind those of cyclases, and only a handful of examples are found in literature (Supporting Information File 1, Table S2). CYPs mostly catalyze prototypical hydroxylations, sometimes followed by

• expression is the most widely used method to study complex terpenoid biosyntheses. Since many bacterial terpenoid BGCs are actinomycete-derived, terpene BGCs are often expressed in model Streptomyces hosts, such as Streptomyces albus, Streptomyces avermitilis, Streptomyces coelicolor, and Streptomyces

• assist in refining our understanding of bacterial terpenoid biosynthesis. Examples of bioactive terpenoids. Repetitive electrophilic and nucleophilic functionalities in terpene and type II PKS-derived polyketide biosynthesis. a) Schematic representation. b) Type II PKS-derived polyketide biosynthesis. c